A luminometer for real-time reporter assay with 8 dish incubator and multicolor measurement system
A luminescence monitoring tool to observe gene expressions with bioluminescent markers under long-term culture. Can be used in studies of internal biological clock, circadian rhythms in organisms, gene silencing, drug stimulation, etc. Kronos Dio is equiped with cell culture system within its housing to provide real-time periodical luminescence measurement with multi-color luciferase assay, enabling users to analyse the effects of complex factors influencing gene expression in the cell or the tissue. Internal temperature control (range: 20 to 45 °C) and carbon dioxide circulation allows for convenient cell culture over a long period of time.
Literature
Published papers which refer to ATTO Kronos Dio are listed below.
- Shin-ya Nishide, Sato Honma, Yoshihiro Nakajima, Masaaki Ikeda, Kenkiti Baba, Yoshihiro Ohmiya and Ken-ichi Honma, New reporter system for Per1 and Bmal1 expressions revealed self-sustained circadian rhythms in peripheral tissues. Genes to Cells 11, 1173-1182 (2006) [Genes to Cells]
- Fujimori Sato, Masatoshi Kurokawa, Nobuhiko Yamauchi and Masa-aki Hattori, Gene silencing of myostatin in differential of chicken embryonic myoblasts by small interfering RNA. Am J Physiol Cell Physiol 291: C538-C545, 2006 [AJP-Cell Physiology]
- Hong Seok Shim, Hyunjung Kim, Jiwon Lee, Gi Hoon Son, Sehyung Cho, Tae H Oh, Sang Hyeon Kang, Dong-Seung Seen, Kun Ho Lee & Kyungjin Kim, Rapid activation of CLOCK by Ca2+-dependent protein kinase C mediates resetting of the mammalian circadian clock. EMBO reports 8, 4, 366-371 (2007) [EMBO reports]
- Tomoya Ohno,Yoshiaki Onishi, and Norio Ishida, A novel E4BP4 element drives circadian expression of mPeriod2. Nucleic Acids Res. 35(2): 648-655. (2007) [Nucleic Acids Research]
- Hiroaki Fukuda, Pei Jian He, Kazuko Yokota, Tomoki Soh, Nobuhiko Yamauchi and Masa-aki Hattori, Progesterone-dependent and -independent expression of the multidrug resistance type I gene in porcine granulosa. Molecular and Cellular Biochemistry. Volume 298, Numbers 1-2, 179-186 (2007) [SpringerLink]
- Pei-Jian He, Masami Hirata, Nobuhiko Yamauchi, Seiichi Hashimoto and Masa-aki Hattori, Gonadotropic regulation of circadian clockwork in rat granulosa cells. Molecular and Cellular Biochemistry. Volume 302, Numbers 1-2, 111-118 (2007) [SpringerLink]
- He, Pei; Fujimoto, Yasunori; Yamauchi, Nobuhiko; Hattori, Masa-aki, Real-time monitoring of cAMP response element binding protein signaling in porcine granulosa cells modulated by ovarian factors. Molecular and Cellular Biochemistry.Volume 290, Numbers 1-2, 177-184 (2006) [IngentaConnect]