Live cell imaging with Ultra-sensitive CCD and Luciferase markers
ATTO Cellgraph AB-3000B is an imaging system designed to detect extremely weak bioluminescent signals from a single cell. In combination with luciferase markers, ATTO Cellgraph can visualize the cell dynamics - time dependent changes of transcriptional activities in individual cells and localization to inner cell components (organelles).
ATTO Cellgraph provides a variety of advanced features to serve living cell studies;
- The ultrasensitive cooled and back-illuminated EMCCD (Electron Multiplying CCD) offers single photon sensitivity, certified with a patented procedure to measure total luminescence intensity as photon counts.
- The incubation system enables long-term culture under controlled temperature and 5% carbon dioxide gas circulation.
- The multicolor detection system is equipped for multi reporter assay with up to 2 different color filters, which enhances sensitivity to extra weak bioluminescence and gives an internal standard basis.
Literature
An original paper by Hoshino, H. et al, which refers to ATTO Cellgraph, was published on Nature Methods in August 2007.
- Hoshino, H., Nakajima, Y., and Ohmiya, Y., Luciferase-YFP fusion tag with enhanced emission for single-cell luminescence imaging. Nature Methods. 4(8), 637-639 (2007) [Nature Methods] / [PubMed]