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AB-2550 Kronos Dio

Bioluminescence measurement system for living cell and tissues
  Kronos  Dio is a luminometer for detecting bioluminescence in living cells and tissues by PMT.

*GPC laboratory CO. LTD. provide useful cell line carrying luciferase as a reporter gene for assessing circadian rhythm by Bmal1 gene expression.

Purpose and Application Product Summary Specifications Documents 

Introducing Kronos Dio operation workflow, including PC software operation as well as experimental protocols(left).
Apoptosis analysis by AB3000 Cellgraph and AB-2550 KronosDio(right)

AB-2550 Kronos Dio Kronos Dio
Purpose and Application
  • Real time reporter gene assay
  •   Transcriptional factor activity,
  •   Clock gene(chronopharmacology).
  •   Gene expression(Transfection,RNAi,etc)
  • Analysis of cell responses
  •   Drug response(Anti-cancer drug,DDS,etc),
  •   Stress response(Hormone,Inflammation,Anti-Oxidant),
  •   Cytotoxichology
  • Analysis of signal pathway
  •   Signal cascade(Calcium),
  •   Apoptosis,etc
Product Summary

Kronos Dio(AB-2550) is a luminometer for detecting bioluminescence in living cells and tissues by PMT. It is available to control temperature and CO2 gas concentration inside Kronos Dio such as an incubator for cell & tissue culturing. Besides Kronos Dio gives superior performance for multi-color assay, which can detect different colors of luminescence up to three in the same time by internal optical filtering system. Kronos Dio is performing to detect high sensitivity for dim and weak luminescence consecutively in culture samples such as cells and tissues in 35mm culture dishes for a long time. Therefore, it is not required to prepare several samples for measuring each time point to chase alteration in chronologic bioluminescence. In this way, it is very reasonable and convenient against conventional way and method in terms of saving time, accuracy and efficiency.

Product Number AB-2550
Product Name Kronos Dio
Container  Culture dish, diameter 35 mm
Number of samples  8
Temperature control  Peltier device and forced air circulation
Temperature range in Kronos Dio incubator   From room temperature – 5°C to 45°C  in increments of 1°C
(Lowest temp.; 20°C )
Temperature control accuracy in Kronos Dio incubator  ±0.5ºC  under room temperature of 25°C
Detector  Photomultiplier tube (PMT)
Measurement method Photon counting method with a photomultiplier tube
Detectable range of wavelength 350 nm to 670 nm
Measurement time  1 to 60 seconds in increments of 1 second
1 to 60 minutes in increments of 1 minute 
Measurement interval  From less than 1 minutes, which depends on time required for eight samples, to 300 minutes in increments of 1 minute
Optical long pass filter  F0: No filter  (whole wavelength)
F1: O56 filter  (cutoff wavelength: less than 560 nm ± 5 nm)
F2: R62 filter  (cutoff wavelength: less than 620 mm ± 5 nm)
Color separation Measurement with automatically selected optical filter up to three colors
CO2 gas precision regulator  Maximum inlet pressure: 1 MPa
Pressure range: 0.007 to 0.07 MPa
CO2 gas controller  Electromagnetic valve
Inlet pressure: -27 to 100 kPa
Outlet pressure: 0 to 50 kPa
CO2 gas sensor  Measurement range: 0 to 20% CO2  (±0.1%)
PC environmental requirements  OS: Windows XP/Vista/7  (32-bit version)
Recommended memory: more than 2GB
HD: Free space of 1 GB or more
Communication USB (Version 1.1) interface
Power AC100-230V 50/60Hz 150W
Dimensions 280(W) × 400(D) × 330(H) mm
Weight 16.0 kg

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